Development of a robust reporter-based ADCC assay with frozen, thaw-and-use cells to measure Fc effector function of therapeutic antibodies
ZJ Cheng, D Garvin, A Paguio, R Moravec… - Journal of …, 2014 - Elsevier
Antibody-dependent cell-mediated cytotoxicity (ADCC) is one of the main mechanisms of
action for many therapeutic antibodies. Classic ADCC assays measure antibody-dependent
target cell cytotoxicity induced by primary effector cells that are isolated from human blood.
They suffer from high assay variability due to the genetic and immune-status-mediated
variation from blood donors. Here we report the development of a robust reporter-based
ADCC assay that uses an engineered Jurkat stable cell line as the source of effector cells …
action for many therapeutic antibodies. Classic ADCC assays measure antibody-dependent
target cell cytotoxicity induced by primary effector cells that are isolated from human blood.
They suffer from high assay variability due to the genetic and immune-status-mediated
variation from blood donors. Here we report the development of a robust reporter-based
ADCC assay that uses an engineered Jurkat stable cell line as the source of effector cells …
