Recombinant Production in Baculovirus‐Infected Insect Cells and Purification of the Mammalian Sec13/Sec31 Complex
C Gurkan, WE Balch - Methods in enzymology, 2005 - Elsevier
Membrane traffic along the eukaryotic secretory pathway starts with the selective packing of
biosynthetic cargo into nascent vesicles that are forming on the endoplasmic reticulum (ER).
This process is mediated by the coat protein complex II (COPII) machinery, which at the
minimum, comprises the Sar1 GTPase and the cytosolic protein complexes Sec23/Sec24
(Sec23/24) and Sec13/Sec31 (Sec13/31). While the components of the basic COPII
machinery are highly conserved from yeast to human, it is now clearly evident that the …
biosynthetic cargo into nascent vesicles that are forming on the endoplasmic reticulum (ER).
This process is mediated by the coat protein complex II (COPII) machinery, which at the
minimum, comprises the Sar1 GTPase and the cytosolic protein complexes Sec23/Sec24
(Sec23/24) and Sec13/Sec31 (Sec13/31). While the components of the basic COPII
machinery are highly conserved from yeast to human, it is now clearly evident that the …
