[HTML][HTML] Standardization and cross validation of alloreactive IFNγ ELISPOT assays within the clinical trials in organ transplantation consortium
I Ashoor, N Najafian, Y Korin, EF Reed… - American Journal of …, 2013 - Elsevier
Emerging evidence indicates memory donor-reactive T cells are detrimental to transplant
outcome and that quantifying the frequency of IFNγ-producing, donor-reactive PBMCs by
ELISPOT has potential utility as an immune monitoring tool. Nonetheless, differences in
assay performance among laboratories limit the ability to compare results. In an effort to
standardize assays, we prepared a panel of common cellular reagent standards, developed
and cross validated a standard operating procedure (SOP) for alloreactive IFNγ ELISPOT …
outcome and that quantifying the frequency of IFNγ-producing, donor-reactive PBMCs by
ELISPOT has potential utility as an immune monitoring tool. Nonetheless, differences in
assay performance among laboratories limit the ability to compare results. In an effort to
standardize assays, we prepared a panel of common cellular reagent standards, developed
and cross validated a standard operating procedure (SOP) for alloreactive IFNγ ELISPOT …